Sequential Loss of CD 34 and Class H MHC Antigens on Purified
نویسندگان
چکیده
The expression of class II MHC and CD34 antigens on human cord blood hematopoietic progenitor cells (HPC) was investigated upon culturing in the presence of interleukin-3 (11-3). HPC isolated by “panning” according to their expression of CD34 coexpressed HLA-DR and HLADP. and the majority of the CD34 HPC also expressed HLA-DQ. In the presence of 11-3. the expression of CD34 and class II MHC antigens was found to be gradually lost in culture. Loss of CD34 expression preceded loss of HLA-DR expression. After eight days of culture. CD34. HLA-DR blast cells were obtained that strongly proliferated in response to 11-3. GM-CSF. G-CSF, and M-CSF, and that had the capacity to generate macrophage and granulocyte colonies. After ten days of culture in 11-3, a population of CD34 cells that expressed low levels of HLA-DR (HLA-
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